*May help reduce biofilm adhesion,
viscosity, and stability*
• May increase the penetration of
antimicrobial molecules into biofilm
complexes*
• Improves the oxidative stress associated
with biofilm formation*

Serrapeptidase, EDTA, trypsin, and lipoic acid to help regulate the effects and integrity of biofilms.* Developed by Darin Ingels, ND, and Allergy Research Group®.

Bacteria often grow within biofilm, a slimy coating that adheres to living tissues.
Biofilm is a thick and sticky substance comprising proteins, polysaccharides and other biomaterials along with divalent cations. This self-made matrix surrounds and shields the microbes from exposure to immune cells and antimicrobial molecules.*
Biofilm complexes are notoriously difficult to disrupt.*
Biofilm Neutralizer*
comprises two orally-available enzymes, trypsin and serrapeptidase, along with alpha-lipoic acid and EDTA (ethylenediaminetetraacetic acid), in a delayed-release capsule (DRCaps®). This mixture is designed to disrupt the bonds that hold biofilm together.*

Serrapeptidase (serratiopeptidase) is a proteolytic enzyme produced by Serratia, a microbe that lives in the digestive tract of various species.* Originally isolated from the silk worm, serrapeptidase allows the emerging moth to dissolve its cocoon.* Purified serrapeptidase has been shown to break down biofilm-associated proteins without harming human tissues.* Serrapeptidase is orally available, and it has systemic fibrinolytic effects.* Trypsin is a proteolytic enzyme made by the pancreas.* It digests a wide range of substrates including bacterial proteins.* Trypsin has been shown to reduce the viscosity and permeability of the biofilm matrix, and to loosen the attachment of biofilm to living tissues.* Additionally, trypsin removes bacterial surface proteins.* Supplemental trypsin is orally available, with the active enzyme appearing in the bloodstream shortly after ingestion.* Alpha-lipoic acid (ALA) is a potent antioxidant found in plants and animals.* Low antioxidant capacity leads to oxidative stress, which triggers microorganisms to shift to a biofilm-producing state.* In clinical trials, ALA has been shown to improve total antioxidant capacity by increasing glutathione, the master antioxidant within cells.* Adequate glutathione levels are associated with healthy immune function.* Additionally, both ALA and glutathione have direct biofilm-disrupting effects.* EDTA Calcium Disodium Salt: EDTA (ethylenediaminetetraacetic acid) is a chelator that binds divalent cations.* Oral EDTA formulations are sometimes used to remove heavy metals, such as lead, from the body.* EDTA loosens the bonds between calcium and alginate, a polysaccharide that strengthens the biofilm matrix. The addition of EDTA enhances the penetration of antimicrobial molecules into biofilm complexes.* The calcium disodium form of EDTA is preferred because it protects serum
calcium levels.*

Suggested Use: As a dietary supplement, 1 capsule one or two times daily between meals, or as directed by a healthcare practitioner.

Caution: EDTA is known to deplete minerals, so repletion is suggested. Higher doses or long-term use require the guidance of a qualified healthcare practitioner with ongoing monitoring of liver and kidney function.

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